个人简介：赵建国，中科院“百人计划”引进国外杰出人才，中国科学院动物研究所干细胞与生殖生物学国家重点实验室研究员，中国科学院大学岗位教授，中国科技大学，安徽大学兼职博士研究生导师。主要研究方向为猪功能基因的筛选，基因组编辑猪疾病模型建立和新品种培育。近年来在PNAS，eLife, Blood, J Mol Cell Biol、Hum Genet等期刊共发表SCI论文30多篇。
Domestic pigs are the second most important source of meat world-wide, and the genetic improvement of economic traits, such as meat production, growth, and disease resistance, is a critical point for efficient production in pigs. Pigs (Artiodactyl family Suidae) lack a functional UCP1 gene, resulting in poor thermoregulation and susceptibility to cold and have a tendency toward fat accumulation. We report application of a CRISPR/Cas9-mediated, homologous recombination (HR)-independent approach to efficiently insert mouse adiponectin-UCP1 into the porcine endogenous UCP1 locus. The resultant UCP1 knock-in (KI) pigs showed an improved ability to maintain body temperature during acute cold exposure with decreased fat deposition. UCP1 KI pigs are a potentially valuable resource for agricultural production through their combination of cold adaptation, which improves pig welfare and reduces economic losses, with reduced fat deposition and increased lean meat production.